Protein Purification

With capabilities of purifying mg to g scale of proteins to >95% purity, with high- throughput using batch- mode or multiple ÄKTA purification platforms in a temperature-controlled environment, we serve a wide range of pharmaceutical and agrochemical clients and academia. Our flexible, fully customizable protein purification services provide highest purity and biological activity with low endotoxin levels at mg to g scale. Having expressed over 200 different proteins, we consistently deliver superior quality proteins as reagents. We have expertise in handling soluble and secretory proteins as well as refolding of synthetic peptide and inclusion bodies. In addition to the purification, modifications like PEGylation, Sortylation, biotinylation, etc. are done in lab scale preparation. We have three well equipped protein purification labs catercatering to process upstream deliverables from microbial, insect and mammalian cell cultures respectively. Each of the labs have dedicated automated purification systems (ÄKTA Start, Go, Pure, Avant), columns, and resins of various chemistries to obtain pure protein and achieve low endotoxin levels.

Diverse column chemistry
  • Affinity- Ni-NTA, GST, FLAG, Protein A/G/L, etc.
  • Ion Exchange- Cation/Anion
  • Mixed Mode Chromatography (MMC)
  • Hydrophobic Interaction Chromatography (HIC)
  • Hydroxyapatite chromatography (HA)
  • Gel Filtration Chromatography (GFC)
Tag removal using various proteases
  • Thrombin
  • TEV protease
  • Enterokinase
  • SUMO protease
  • Precision protease
Solubilization & refolding of inclusion bodies
  • Urea
  • GnHCl
  • Refolding Screens
    • Commercial screens
    • Customized screens (pH, buffer, redox systems, additives etc)
  • Biotinylation (in vitro, in vivo)
  • PEGylation
  • Sortylation