Protein Characterization
At Cell and Protein Sciences, we offer a comprehensive range of quality analysis services for purified recombinant proteins. Our standard protein analysis package includes SDS-PAGE, Western blot, aSE-HPLC, and intact mass analysis using mass spectrometry. Additionally, we customize our services to meet customer requirements, offering specialized protein characterization such as peptide mass fingerprinting (PMF) and PTM analysis using MS/MS, NCE and NBE affinity analysis using SPR, protein stability analysis, charge – variant analysis, and more.
Our range of protein characterization services includes:
Infrastructure
- Biacore™ T200 SPR system (Affinity)
- Agilent 6545XT AdvanceBio LC/Q-TOF System (Mass and identity)
- QuantStudio™ 5 Real-Time PCR System (Stability)
- Agilent 1290 Infinity II UHPLC System (Purity, Quantity, and Integrity)
- Agilent 1260 Infinity II HPLC System (Purity, Quantity, and Integrity)
- Shimadzu N-series XS UHPLC system (Purity, Quantity, and Integrity)
- SpectraMax iD5 Multi mode plate reader (Absorbance, Fluorescence, and Chemiluminescence)
- Endosafe® nexgen-PTS™ (Endotoxin detection)
- BD FACS Aria™ Fusion Flow Cytometer (Immunoreactivity)
- ProteinSimple WES and iBlot™ 2 Dry Blotting System (Purity and Identity)
Our Facility
Frequently Asked Questions (FAQs)
Aragen's standard protein analysis package covers SDS-PAGE, Western blot, aSEC-HPLC, and intact-mass analysis by mass spectrometry, with add-ons including peptide mass fingerprinting, PTM analysis, SPR-based affinity analysis, and charge-variant analysis using a Biacore T200 SPR system and an Agilent LC/Q-TOF.
