Molecular Biology

At Aragen’s Cell and Protein Sciences, we provide a full range of molecular biology services customized to your specific needs such as, gene synthesis, sub-cloning, vector construction, sequence analysis, plasmid DNA preparation and scale-up, and gene expression analysis. By partnering with us for your molecular biology needs, you’ll gain access to an extensive portfolio of state-of-the-art services and proven expertise, all designed to enhance the speed, precision, and impact of your work.

Gene Synthesis & Sub-Cloning 

• Codon-optimized gene synthesis to suit your choice of recombinant protein expression system
• Sub-cloning into desired expression vector with the option to introduce signal sequences, solubility – enhancing domains, custom tags, protease digestion sites, linkers, etc. 
• Diverse cloning strategies- ligation-dependent, independent cloning (In-Fusion cloning, Gibson assembly, Gateway cloning)
• Vector construction customized to client specifications
• Site-directed mutagenesis

Plasmid DNA Scale – Up (µg – mg)

• High purity endotoxin free plasmid DNA scale up
• Scale – up range: Mini prep, Midi prep, Maxi prep, Mega prep, and Giga prep

Other Specific Services:

• Phage display technology 
• V–gene sequencing

Timelines

Gene synthesis: ~ 2 – 3 weeks

Sub-cloning: ~ 5 days

Plasmid DNA scale-up: 3 days