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Protein Characterization

Aragen has a strong team of scientists highly qualified to do protein characterization. The Analytical lab is equipped with state-of-the-art equipment like Biacore T200, UHPLC, Spectrophotometer, Quantstudio 5 etc. to carry out the protein analysis. We have the complete suite of characterization to qualify the proteins produced at Aragen. All proteins that are produced undergo stringent QC check before shipped to client site.

Quantitation
  • A280
  • Bradford assay
  • BCA assay
  • PA-HPLC (Antibodies)
Purity
  • SDS-PAGE (Reducing, non-reducing, native)
Aggregation
  • aSE-HPLC
  • Thermal shift assay
Charge variant analysis
  • IEC-HPLC
Immuno reactivity
  • Western blotting
  • ELISA
  • FACS
Affinity
  • SPR (Biacore)
Identity
  • Intact mass determination
  • Reduced deglyc mass
  • Peptide Mass Fingerprinting (PMF)
HCP
  • ELISA
Host Cell DNA
  • qPCR
Endotoxin Measurement
  • LAL assay (Kinetic)
  • PTS Cartridge