In Vitro

The nature of the molecule, the biology being investigated, and the overall requirements for throughput and yield, determine the in vitro testing decision. Aragen skilled scientists work with customers to develop and implement assays best suited for their specific research requirements. We offer a diverse portfolio of cell-based and biophysical assays that enable the development and characterization of new therapeutics, based on the physical characterization of the protein (expression, size, weight, binding), as well as biological activity in mammalian cells, and ultimately, in animals.

Our cell biology team has extensive experience in developing cell-based assays for determining mechanisms of action, evaluating potential therapeutics, and screening small libraries of compounds. The protein sciences team provides a range of analytical methods using multiple assay platforms to characterize therapeutic, diagnostic or reagent-grade proteins.

Areas of ExpertiseAssay Type
  •  Immunophenotyping
  • Lymphocyte subset analysis
  • Toxicity assays – ADCC, ADCP, CDC
  • Cell activation
  • Fc receptor binding
  • Mixed lymphocyte reactions
  • Cytotoxic T lymphocyte (CTL) and natural killer activity assays
  • Treg, NK cell, macrophage assays
  • Multiplex cytokine profiling
  • ELISpot
  • Immune function assays
  • Live-cell imaging using the IncuCyte S3


  • Apoptosis
  • Cell proliferation (BrdU, MTT)
  • Antibody-target affinity
  • In vitro angiogenesis
  • Phospho-flow
  • Reporter gene assays
  • Migration assays
  • Custom assays
Primary Cell
  • Immunology assays
  • Other (a variety of primary immune cells isolated from whole blood or buffy coats)
Fibrosis assays
  • Epithelial-to-mesenchymal transition (EMT)
  • Fibroblast-myofibroblast transformation (FMT)
  • Co-culture assay
  • Precision-cut tissue slice assay
SARS-CoV-2 screening assay
  • Pseudovirus system
  • Genotyping
  • siRNA testing
  • Chemotaxis assays: employ a Transwell containing a membrane with a defined pore size; run in 96-well format
  • Cell migration and cell invasion assays: employ ECM degradation and proteolysis
  • Cell adhesion assays
  • Custom assays