Accelerating Oligonucleotides Discovery and Development Programs

Aragen embarks on oligonucleotide service with experience to construct novel oligonucleotide, characterize and conduct biological evaluation.
Aragen Life Sciences has established an advanced state-of-the-art laboratory for synthesis of quality oligonucleotides, from simple to highly complex oligonucleotides for diverse applications viz. ASO, siRNA, miRNA, Aptamer, and other niche applications. A highly talented and experienced scientific team to handle modern automatic synthesis, purification, and characterization of modified oligonucleotides.
In addition, Aragen can support synthesis of customized building-blocks for oligonucleotide synthesis. Aragen has strong track record in delivering high-quality nucleoside/tide synthesis and formulation services to partners worldwide for over a decade. There is a dedicated in-vitro and in-vivo laboratory to support oligonucleotides discovery programs. Our expertise and commitment have been validated by our customers across the globe, as we create these molecular structures for businesses worldwide always with an emphasis on quality and reliability

Process flow for the synthesis of oligonucleotides

Oligonuceotide synthesis portfolio

  • Standard: DNA, RNA, 2’OMe-RNA, 2’F-RNA, 2’MOE-RNA, LNA
  • Custom synthesized sugar and base modified nucleotides
  • Linkers: PO/PS/P-Me chemistry
  • Dynamic synthesis scale flexibility: 1-100 umol
  • Yield scale: 1-100 mg
  • (3’/5’/int)
Functional units:
  • Fluorophores, quenchers, cholesterol, biotin,
    digoxigenin, tris-galnac, phosphate,
    5’-vinyl-phosphate, lipids spacers and others
Attachment units:
  • amine/thiol/alkyne/azide
  • Bulk-scale primers and probes
  • Small oligonucleotides (5-15 nt)
  • Antisense oligonucleotide (16-22 nt)
  • siRNAs (20-35 nt)
  • Aptamers (35-75 nt)
  • Long oligonucleotide for RNA editing and other applications
Solution phase conjugation:
  • Acid-amine coupling
  • Thiol-maleimide coupling
  • Azide-alkyne coupling
In-house synthetic expertise to synthesize
modified bases, linkers, and sugar motifs

Modern equipment’s for synthesis, analysis, purification, characterization and sample processing

Oligonucleotide Synthesizer: H-16, K&A

Phenomenal flexibility, ultra-low maintenance, short cycle times, and lowconsumption featured with 2 columns to 16 columns for wide range of scales oligonucleotide synthesis.

  • Automatic Recipe Management for 16 channel
  • Online trityl monitor for all columns
  • 12 amidite positions and 9 reagent positions for synthesis of highly
    complex oligonucleotide
Oligonucleotides Purification:
  • Anion exchange purification with AKTA Pure 150M3
  • Reverse-Phase HPLC purification with Shimadzu HPLC system
Characterization & Sequencing:
  • UV based quantification of final product
  • Purity analysis: Acquity Premier UPLC with Ion-pairing RP-HPLC mode
  • HRMS: Thermo Orbitrap Exploris 240 Mass Spectrometer for mass confirmation
  • Sequencing capability: MS/MS studies to identify and quantify impurities along with sequence confirmation