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Peptide Technology Platform

Chemistry Expertise

Protein synthesis ( > 150AA) in gram scale
Linear, branched, cyclic and stapled peptides
40-50 AA, mg to gm, Peptide libraries
Solid, liquid, hybrid, TAG-assisted, native chemical/KAHA ligation
C- & N-terminal, cyclization, backbone
modifications, late-stage functionalization,
and conjugation reactions
Non-GMP scale-up
Purification and characterization

Biology Expertise

Peptide mapping/ fingerprinting
Plasma protein binding using Rapid Equilibrium Dialysis or Ultrafiltration
Stability in buffer, simulated gastric fluids, liver, kidney, intestinal S9, hepatocytes or plasma
Half-life, metabolite identification and profiling
Pharmacokinetics and Quantitative bioanalysis: rat, mouse, dog, rabbit
Mass/ QTOF spectrometry analysis

Track Record of Success

Library development: linear/cyclic peptides, 2-20 mg, up to 20mer, >90% purity
Successful synthesis of challenging peptide moieties
Hydrophobic peptides with disruptors (dmb, hmb protected amino acids / pseudoprolines, chaotropic agents)
Cyclic peptides (S-S bridging, cyclization, stapling, RCM, thioether)
Process optimization and non-GMP scale-up to multi-gm scale (>500g, 98% pure, single impurity <0.5%)
Modified interleukin protein synthesis (>150 amino acid, >10g scale) using ligation techniques (NCL/KAHA)
Long chain peptides and folding

World Class Infrastructure

Microwave assisted peptide synthesizer- Liberty blue
CEM-multipep-2 parallel peptide synthesizer
Parallel automated synthesizer with online UV- Symphony X
Automated scale-up peptide synthesizer- Sonata
Up to 10 L reactors for scale-up
Lyophilization at scale (Tree and tray lyophilizers)

Analytical Capabilities

Peptide characterization by UV, LCMS, HRMS and NMR
Purification by RP Preparative HPLC (2000 to 80000 Da)
Up to100mm dia DAC column for scale-up purification custom peptides and peptide libraries
Quality control of all custom peptides and peptide libraries