Virus Production

Viral vectors (Lentiviral and Adeno-associated virus) are the most common gene delivery tools for hard to transfect and non-dividing cells. Cell and Protein Sciences provides wide range of viral vector production and testing services for preclinical studies.

Lentivirus (LV)

Lentivirus is a type of retrovirus containing single-stranded RNA genome. Lentivirus has been widely used as gene delivery tool for permanent integration of target gene into cells. We have capability to produce research grade high quality lentivirus.

Why Aragen?

• Optimized 3rd generation lentivirus generation platform
• High-quality replication-incompetent virus
• Titer determination by qPCR
• Titer >107 IFU/mL leading to high transduction performance
• Mycoplasma tested
• Quality testing for expression by Western blot/ ELISA/ FACS

Timeline: 3-4 weeks*

* Excluding gene synthesis 

Adeno-associated virus (AAV)

AAV is a non-enveloped, single-stranded DNA virus that can deliver the gene to both dividing and non-dividing cells. AAV vectors can support selected research and development applications. Aragen supports research-grade AAV particle production and testing based on project requirements.

Why Aragen?

• Optimized platform to produce multiple serotypes
• High quality AAV particles
• In-house AAV plasmid preparation and gene validation by sanger sequencing
• Genomic titer determination by qPCR
• Mycoplasma tested

Timeline: 3-5 weeks*

* Excluding gene synthesis