Cell Line Development

At Cell and Protein Sciences, we offer comprehensive cell line development services designed to streamline your workflow from cell generation to high-yield production of monoclonal antibodies, recombinant proteins, and viral vectors. Our teams use established cell line development workflows and analytical methods to support stable cell line generation. 

We ensure that our cell lines undergo rigorous testing for all quality parameters before they are shipped to clients. Additionally, we offer culture condition optimization services using advanced media and feeds to maximize yield and product quality. Cell banks are generated and maintained under controlled conditions, with quality control measures including mycoplasma testing and assessments of cell viability and stability.

Our full suite of cell line development services includes:

Tool cell line background: CHO, HEK293, Cancer cell lines, non-human cell lines

Production cell line background: CHO-S, ExpiCHO-S, HEK293-F, Expi293-F                                                                             

Promoters: CMV, EF1α or any natural/synthetic promoter for reporter cell lines

Selection markers: G418, Hygromycin, Puromycin, Blasticidin, Zeocin

Reporter genes: GFP, YFP, RFP, luciferase, HiBiT, HALO

Mode of expression: Intracellular, secretory or membrane

Gene delivery tools: 

• Chemical transfection (Lipid-based, PEI-based)
• Electroporation (Neon electroporation, Amaxa 4D-nucleofector) 
• Viral transduction (Retrovirus, Lentivirus)

Quality analysis: 

• Expression levels (Western blot, FACS, Fluorescence, Luminescence, qPCR)
• Mycoplasma testing
• Sterility test
• Freeze-thaw test
• Stability analysis

Timeline: 

Tool cell line: Stable pool: 4-6 weeks*; Monoclones: 10-12 weeks*

Production cell line: Stable pool: 6-7 weeks*; Monoclones: 14-18 weeks*

*Excluding gene synthesis