Oligonucleotide

Accelerating Oligonucleotides Discovery and Development Programs

With a commitment to innovation and excellence, Aragen offers research and development (R&D) scale oligonucleotides and oligonucleotide conjugate synthesis services. Our laboratory supports synthesis of simple to complex oligonucleotides for applications such as ASO, siRNA, miRNA, aptamers and other research applications. An experienced scientific team supports automated synthesis, purification and characterisation of modified oligonucleotides.

Service Offerings:

• DNA Oligonucleotides
• RNA Oligonucleotides
• Oligonucleotide conjugates
• Synthesis of customized building-blocks for oligonucleotide synthesis

Process flow for synthesis of oligonucleotides

Project evaluation

Comprehensive analysis of the project feasibility and goals by our experts.
Estimation of raw material requirements, costs, and timelines

Purification

Reverse-phase HPLC
purification, Anion
exchange purification,
Desalting

Delivery

Sharing of final analytical data
Shipping of final product according to your request

Project request

Customized discovery and synthesis requests from researchers across the globe

Synthesis

Synthesis scale: 1 μmol – 500 μmol, Yield scale: 1 mg – 500 mg (deliverable quantities), oligonucleotide lengths: 5 – 10 mer

Quality assurance

RP-HPLC purity check (UV), MS confirmation, Quantification based on A260 (UV-mg)

Our portfolio

• Nucleotides:
  • Standard: DNA, RNA, 2’OMe-RNA, 2’F-RNA, 2’MOE-RNA, LNA
  • Custom synthesized sugar and base modified nucleotides
  • Linkers: PO/PS/P-NHMs chemistry
• Scale:
  • Dynamic synthesis scale flexibility: 1-500 μmol
  • Yield scale: 1-500 mg
• Applications:
  • Primers and probes
  • Small oligonucleotides (5-15 nt)
  • Antisense oligonucleotides (16-22 nt)
  • siRNA oligonucleotides (20-35 nt)
  • Aptamers (35-75 nt)
• Modification:
  • 3’/5’/int
• Functional units:
  • Fluorophores, quenchers, cholesterol, biotin, digoxigenin, phosphate
  • 5’-vinyl-phosphate, lipids, spacers and others
• Conjugation chemistries:
  • Acid-amine coupling
  • Thiol-maleimide coupling
  • Azide-alkyne coupling
• Cross department services
  • In-house synthetic expertise to synthesize modified bases, linkers, and sugar motifs
  • Oligonucleotide bioassays: tox studies, efficacy studies

Oligonucleotide Infrastructure

Our in-house synthetic expertise is supported by automated synthesis, purification and analytical workflows.

Synthesis

• 16 channel oligo synthesizer with 12 channels for amidite building blocks from K&A 
• 16 oligonucleotide synthesis upto 10 μmol scale; 250 oligos per month
• Chimeric or patterned mixture of PO/PS backbone modification

Purification 

• Ion exchange HPLC purification 
• Ion-pair reverse-phase HPLC purification
• Desalting using sephadex G25, MWCO spin filtration or C18 cartridge

Analysis

• A260 based quantification using nano spectrophotometry
• UPLC purity and HRMS mass confirmation
• Bacterial endotoxin analysis
• Duplex formation analysis

Shipping formats

• Lyophilized solid
• Formulated in buffer of choice
• Aliquoting